最新刊期

      ZHANG Xiaoying, XIE yuquan, ZHANG yuchan, SI qi, XIAO yuanhu, GUO yuqi, SHAO xiayu

      DOI：10.11714/jsysu.med.YX20260105
      摘要：ObjectiveTo investigate the protective effect and underlying mechanisms of the Bawei Chenxiang Wan （BCW） against myocardial ischemic injury.MethodsMyocardial ischemia was induced in rats by subcutaneous injection of isoproterenol （ISO）， and ex vivo ischemia was established by coronary artery ligation in isolated rat hearts.The effects of BCW and its polar extracts on electrocardiogram （ECG）， myocardial histopathology， serum cardiac enzymes， hemodynamic parameters， and infarct size were evaluated. The expression of proteins related to the PI3K and cuproptosis （FDX1， LIAS， DLAT） were detected. The levels of Cu²⁺and glutathione in myocardial tissue were measured. In an oxygen-glucose deprivation （OGD） model of H9c2 cardiomyocyte injury， the effects of the n-butanol extract of BCW and the PI3K agonist and inhibitor were examined.ResultsBCW ameliorated ST-segment abnormalities on electrocardiograms， reduced serum levels of myocardial injury markers， improved hemodynamic parameters， upregulated PI3K phosphorylation（P＜0.01）， and decreased Cu²⁺ accumulation in rats with myocardial ischemia（P＜0.01）. Moreover， BCW reduced the myocardial infarct size. In the isolated rat heart model of ischemia， both the ethanol extract and various solvent fractions of BCW exerted protective effects and reduced Cu²⁺ content， among which the n-butanol fraction exhibited the most potent activity（P＜0.01）. Specifically， the n-butanol fraction upregulated PI3K phosphorylation（P＜0.01） and downregulated the expression of cuproptosis-related proteins， including FDX1（P＜0.05）， LIAS， and DLAT oligomerization（P＜0.05）. In the OGD-induced cardiomyocyte injury model， treatment with a PI3K agonist inhibited DLAT oligomerization（P＜0.05）， whereas co-administration of a PI3K inhibitor attenuated the inhibitory effect of the n-butanol fraction on DLAT oligomer formation（P＜0.05）.ConclusionBCW exerts a significant protective effect against myocardial ischemic injury， and its mechanism may involve activation of the PI3K and inhibition of cuproptosis.  
      关键词：Bawei Chenxiang Wan;myocardial ischemia;n-butanol extract;PI3K;cuproptosi;dihydrolipoamide S-acetyltransferase   
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