【Objective】To detect the positive rate and number of circulating tumorcells（CTC）inperipheral blood of healthy women，benign epithelial ovarian tumors and patients with ovarian malignante pithelial cancer（includingallstages），and to preliminarily evaluate the clinical significance of CTC detection in ovarian malignan tepithelial cancer screening.【Methods】This project was achieved by collecting 151 female controls（normal group），138 cases of benign ovarian epithelial tumor（benign group）and 92 cases of ovarian epithelial cancer（epithelial ovarian cancer including all types and clinical stages）patients（malignant group），sent from Zhongshan Boai Hospital and Zhongshan Hospital from January 2016 to December 2017. Patients with ovarian cancer were detected by gold pathological standard. Venous blood samples were collected before surgery in each group. Negative enrichment of immunomagnetic beads and immunofluorescence in situ hybridization were using. Then we compared positive rate of CTC inperipheral blood and the number of differences；statistical analysis CTC detection and diagnosis ovarian epithelial cancer，sensitivity，specificity，accuracy，positive predictive value and negative predictive value，the value of comprehensive evaluation of CTC detection in the diagnosis of ovarian epithelial cancer. 【Results】①The positive rates of CTC in the normal group，the benign group and the malignant group were 0，0 and 90.22%，respectively. The overall difference was statistically significant（P<0.001）；The difference between the malignant group and the normal group，the malignant group and the benign group was statistically significant（P<0.001） . ②The positive rates of CTC instageⅠ-Ⅳgroup were 81.25%，89.47%，90.32% and 96.15%. The difference was not statistically significant（P=0.47） . ③The average number of CTC in the normal group，the benign group and the malignant group were 0，（0.02±0.15），（8.27±10.88）. The total difference was statistically significant （P<0.001）. There was no difference between the benign group and the normal group（P=0.97），but were significant differences between the malignant group and the benign group，the malignant group and the normal group（all P <0.001） . ④The mean CTC in stageⅠ-Ⅳ of malignant group were（2.13±1.31）， （2.68±1.53）， （5.90±2.95），and（19.35± 15.19），respectively. The difference was statistically significant（P<0.001）.There was no significant difference between stage Ⅲ and stage Ⅱ（P=0.19），stage Ⅲ and stage I（P=0.14），stage II and stage I（P=0.84），and there stperiods were statistically significant（P <0.001） . ⑤The sensitivity，specificity，and accuracy of CTC diagnosis in ovarian epithelial cancer，were 90.22%，100%，and 97.64%. 【Conclusion】The positive rate and the average number of CTC in ovarian epithelial cancer patients are the highest，and the mean number of CTC is correlated with the clinical stage of ovarian epithelial cancer. It indicates that CTC has high clinical value in the early diagnosis and clinical stage prediction of ovarian epithelial cancer.