： 【Objective】To investigate the characteristics of immunophenotypes in hepatocellular carcinoma（HCC） before liver transplantation.【Methods】The immunophenotypes of T-，B- cells，monocytes，dendritic cells（DC）and NK-cells in peripheral blood from 6 HCC patient who were ready to have liver transplantation and 6 healthy volunteers were analyzed by multicolor flow cytometry.【Results】In the patients，the proportions of CD4 + PD-1 + T cells，Treg cell （CD4 + CD25 + CD39 + T cells），CD19 + B cells，Plasmablasts（CD27 high CD38 high IgD - IgM - ），classical monocytes（CD14 high CD16 - ）and mature NK-cells（CD3 - CD56 high ）were all higher than those in the healthy controls（all P<0.05）. However， marginal zone B cell（CD27 + IgD + ），Non-switched B cells（CD27 + CD38 dim IgM + ），intermediate monocytes（CD14 highCD16 + ）and immature NK-cells（CD3 - CD56 + ）were lower than those in the healthy controls（all P<0.05）. And there wasn’t any obvious difference in quantity being observed among other cell types.【Conclusion】There was difference in the immunophenotypes of immune cells in peripheral blood between HCC patients before liver transplantation and healthy people. And this finding exerts important effects on monitoring the immune status of the patients after liver transplantation and guiding the administrations of immunosuppressors.