【Objectives】To investigate the protective effects of recombinant Trichinella spiralis excretory-secretory 53ku pro? tein（rTsP53）on acute lung injuries in mice.【Methods】Thirty Balb/c mice were randomly divided into normal group. ALI group and rTsP53 group（n = 10，respectively）. Macrophages were harvested by bronchoalveolar lavage. Mortality in 72 hours was counted and compared. Pathological damage of lung tissues was observed by HE staining and graded by Smith score. Wet/dry ratio was measured. The bronchoalveolar lavage fluid concertration of IL-6 and IL-4 was measured by ELISA. The mRNA expression of TNF-α，iNOS， IL-10 and Arg-1 in alveolar lavage macrophages was detected by RT-PCR.【Results】72 h mortality of ALI mice was 70%，which was reduced to 30% in mice received rTsP53 treatment. Compared with ALI mice，the pathological damage of in rTsP53 treated- mice was improved and Smith score was declined，combined with descending W/D ratio. IL-6 level of alveolar lavage fluid was elevated in ALI mice compared with normal group. And alveolar lavage macrophage was polarized to M2 sub-type，appeared as higher mRNA expression of TNF-α and iNOS and lower level of IL-10 and Arg-1. Bronchoalveolar lavage fluid concentration of IL-6 was declined and IL-4 was elevated in rTsP53-treated mice compared with ALI group. The macrophages of alveolar wash hadhigher mRNA expression of IL-10 and Arg-1，while lower level of TNF-α and iNOS，manifesting M2 polarization characteristics. 【Conclusion】Recombinant T.spiralis P53 protein could protect mice from acute lung injuries induced by LPS via modulating M2 macrophage polarization，which play a role in depression of inflammatory reaction and tissue repairment.