Abstract： 【Objective】To investigate the effects of ghrelin on inflammatory signaling protein kinase B（Akt），nuclear factor-κB（NF-κB）and inducible nitric oxide synthase（iNOS）in alveolar macrophage（AM）.【Methods】24 Male SD rats were randomly divided into Sham，CLP，CLP+ghrelin，and Sham+ghrelin groups. Cecal ligation and puncture（CLP）was used to induce sepsis. Ghrelin（20 nmol/kg）was administered by intraperitoneal injection at 3 h and 15 h post-operation. Histopathological changes of lungs were observed and scored. AM were extracted from bronchoalveolar lavage fluid（BALF）. Interleukin-1β（IL-1β），tumor necrosis factor-α（TNF-α）and interleukin-6（IL-6）in BALF were detected by ELISA. IL-1β，TNF-α，and IL-6 mRNA in AM were detected by qPCR. NF-κB p65，IκBα，p-IκBα，Akt，p-Akt and iNOS in AM were detected by immunofluorescence（IF）and Western blotting.【Results】The histologic score（6.7±0.8），BALF IL-1β［（146±12）pg /mL］and IL-6［（182±10）pg/mL］from CLP+ghrelin group were respectively 35.4%，44.5% and 46.42% lower than those from CLP group［（10.3±0.7），（263±17）pg/mL，and（273±5）pg/mL］，P<0.05. No significant difference was found in BALF TNF-α between CLP group and CLP+ghrelin group. The IL-1β，TNF-α and IL-6 mRNA in AM from CLP+ghrelin group were respectively 54.38%，53.6% and 46.42% lower than those from CLP group，P<0.05. The nuclear NF-κB p65 and cytoplasmic p-IκBα，p-Akt and iNOS from CLP+ghrelin group were respectively 32.58%，45.42%，27.6% and 48.33% lower than those from CLP group，P<0.05. There was no significant difference in all data between Sham group and Sham+ghrelin group.【Conclusion】Ghrelin can decrease the activity of inflammatory signaling proteins Akt，NF-κB and iNOS in AM，therefore restricts AM expressing pro-inflammatory cytokines IL-1β，TNF-α，and IL-6，thus alleviates sep? sis-induced acute lung injury（ALI）.