【Objective】To investigate the possible effect of microRNA-374（miR-374）expression on tumor cells′ proliferation and invasion and particular mechanism.【Methods】MiR-374 overexpression lentiviral vector（Lv-miR-374）and a control lentiviral empty vector（LEV）were stably transfected into human glioma U251 and U87 cells，to evaluate the effect of miR-374 on cell prolifer? ation and invasion ability. Target relationship between miR-374 and PTTG were researched by dual luciferase report gene assay. Ex? pression level of correlative signaling pathways of the downstream gene protein was analyzed by Western blot.【Results】We revealed that the overexpression of miR-374 dramatically suppressed glioma cell growth and invasion in vitro. Target relationship between miR- 374 and PTTG was confirmed by dual luciferase report gene assay. And decreased protein expression of PTTG，bFGF，AKT，MMP2， and p70S6K was consistent with the effect of miR-374 overexpression.【Conclusion】Decreased miRNA-374 is an unfavorable progno? sis marker and promotes glioma cell growth and invasion via direct targeting PTTG. Our findings provide new insights into the role of miR-374 in the development of gliomas，and implicate the potential application of miR-374 in cancer therapy.