【Objective】To compare the single live birth outcomes of blastocyst transfer between vitrified blastocyst and blastocyst cultured from thawing cleavage embryo，so as to choose the best scheme of blastocyst transfer.【Methods】 Retrospective analysis of the single live birth clinical data of 1 037 vitrified blastocyst compared with 690 blastocyst cul? tured from thawing cleavage embryo undergoing frozen embryo transplantation（FET）from January 2014 to October 2016 was performed. Mail outcome were including gestational age，neonatal weight，proportion of male neonate，preterm birth rate，very preterm birth rate，low birthweight rate，very low birthweight rate，congenital anomalies rate.【Results】There were no differences between the two groups for gestational age，neonatal weight，proportion of Live birth，health baby and stillbirth（P>0.05）. There were no differences in proportion of male neonate（AOR 1.07，95% CI 0.86~1.34），preterm birth rate（AOR 0.7，95% CI 0.49~1.01），very preterm birth rate（AOR 1.47，95% CI 0.55~3.96），low birthweight rate （AOR 1.38，95% CI 0.86~2.22），very low birthweight rate（AOR 0.76，95% CI 0.20~2.83），congenital anomalies rate （AOR 1.58，95% CI 0.66~3.76，P>0.05）.【Conclusion】The blastocyst may be the preferable stage for vitrifying andtransfer currently which can obtain good neonatal outcomes.