【Objective】To explore the effect of HSYA on pro/anti-inflammatory cytokine′s levels and mRNA expression in peripheral blood of mice.with sepsis.【Methods】Dividing NIH mice into four groups，as normal group，sham group，CLP group and HSYA group，24 mice in each group. The CLP sepsis mouse model was established. HSYA（120 mg/kg）were injected intravenously at 12 h before the operation，and 0 h and 12 h following CLP，and other groups were given normal saline. observed the animals behavior changes，measured the levels WBC，PLT，ALT，AST，BUN in serum，detected the levels and mRNA expression of IL-6， IL-10，TNF-α in peripheral blood. cultured of peripheral blood bacteria loads.【Results】24 h after surgery，mice in CLP group appeared furring，feces residues on anus etc. compared to normal group，sham group and HSYA group，WBC，PLT，ALT，AST， BUN，levels and mRNA expression of IL-6，IL-10，TNF-α showed significant increases，it was also found that bacterial load was significant increased in model group.【Conclusions】HSYA has a therapeutic effect in mice with sepsis，can reduce bacteria into the blood，and inhibit inflammatory mediators which caused tissue damage.