【Objective】Exploring the expression of breast cancer related miRNA in the serum of normal population and breast cancer patients

analysis its clinical value in the diagnosis of breast cancer. 【Methods】 Serums were collected from normal population and breast cancer patients. RT-PCR was used to test the expression of miR-630

miR-21

miR-195

miR-134

miR-200a

miR-381 and miR-1228.The value of single serum miRNA in diagnosis of breast cancer were analyzed

the diagnostic value of combined detection of multiple miRNA were further analyzed by using statistical methods.【Results】Compared to normal population

serum miR134 and miR21 were increased in breast cancer patients

P<0.01; the expression of miR-134 had no difference between the early stage breast cancer patients and late stage breast cancer patients

P=0.143; the expression of miR-21 increased in the late stage breast cancer patients

P=0.004. Meanwhile

the expression of miR-195

miR-630

miR-200a

miR-381

miR-1228 levels between the two groups had no statistical significance. ROC curve analysis showed that

in the diagnosis of breast

AUC value of miR-21 was 0.82 (95% CI

0.71-0.88)

P<0.001; AUC value of miR-134 was 0.88 (95% CI

0.80-0.94)

P<0.001; AUC value of miR-21 combined with miR-134 in the diagnosis of breast was 0.95 (95% CI

0.89-0.99)

P<0.001

which was better than a single detector miR-21 or miR-134

P<0.05.【Conclusion】The expression of serum miR-21 and miR-134 in the breast cancer patients was elevated and miR-21 combined with miR-134 can be used as an ideal marker for the diagnosis of breast cancer.