【Objective】To investigate the characteristics of PD-1/PD-L1 expressed on T cell and bladder cancer cell and clini? cal significance.【Methods】64 patients with primary bladder cancer were into experiment group and 10 normal people were into con? trol group. Peripheral bloods were used to test the PD-1 expressed on CD8 + T lymphocytes by flow cytometry. Immunohistochemistry staining was used to detect the PD-L1 expression in tumor and normal specimen.【Results】PD-1 expressed on CD8 + T lymphocytes was（2.25 ± 0.60）% in experiment group and（0.68 ± 0.17）% in control group，respectively（P < 0.001）. And the PD-1 expression on T cell in invasive bladder cancer patient was significant higher than superficial bladder cancer patients［（3.04 ± 0.46）% vs（0.68 ± 0.17）%，P < 0.001］. The expression of PD-L1 in experiment group was higher than control group，（26/64 vs 0/15，P < 0.001）. But there was no different between invasive and superficial bladder cancer patients，（41.3% vs 38.8%，P > 0.01）.【Conclusions】Ex? pression of negative stimulatory molecule PD-1 in CD8 + T lymphocytes of peripheral blood is significantly correlated with bladder can? cer advanced. Bladder cancer cell was strongly expressed PD-L1，and this expression is not related to cancer advanced.