【Objective】To radiolabel the PSMA aptamer A10-3.2 with 99m Tc

and explore its biological characteristics in vivo and in vitro.【Methods】Using Succinimidyl 6-hydrazinonicotinate hydrochloride (SHNH) as the bifunctional chelating agent to label aptamer A10-3.2 with 99m Tc

then tested for the stability in vitro

the specific uptake by prostate cancer LNCaP cells (PSMA+)

the characteristics of SPECT/CT imaging and biodistribution in LNCaP tumor-bearing NOD/SCID mice.【Results】The labeling rate and radiochemical purity of the products ( 99m Tc-SHNH-A10-3.2) are（71.31±6.78）% and 97.03%

respectively. 99m Tc-SHNH-A10-3.2 had obvious target specificity for PSMA positive prostate cancer LNCaP cells

its uptake rate was significantly higher than PSMA nega? tive PC-3 cells (P<0.01). And in tumor-bearing mice

the tumor has a certain uptake and a high ratio of the tumor tissue to the mus? cle.【Conclusion】This study successfully constructed 99m Tc-labeled PSMA-targeted aptamer A10-3.2

which has a good stability and targeting in vivo and in vitro

has a high tumor tissue/muscle ratio in tumor-bearing mice

which show that it may be a potential target? ed molecular imaging agent for prostate cancer.