网络首发:2015-12-20,
纸质出版:2015
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自制新型吸引头在自体血液回收中的应用[J]. 中山大学学报(医学科学版), 2015,36(6).
Use of A New Type Suction Tip in Red Blood Cell Salvage[J]. Journal of Sun Yat-sen University (Medical Sciences), 2015, 36(6).
摘 要: 【目的】 比较自制新型吸引头和传统吸引头在不同出血速度下对回收血液中红细胞溶血率的影响,探讨新型吸引头的临床实用性。 【方法】 收集骨科手术术后6 h内引流血90例,随机分为2组:A组(传统吸引头组),B组(新型吸引头组),各45例。各组再根据出血速度不同分为3个亚组,其中A1、B1组(V出血 = 2 mL/min),A2、B2组(V出血 = 4 mL/min),A3、B3组(V出血 = 8 mL/min),每个亚组15例,共6个亚组。建立体外负压抽吸模型,维持负压大小-0.02 MPa,调节抗凝剂滴速V = 1 mL/min。负压抽吸5 min后采集血样,通过检测血样中游离血红蛋白浓度计算回收血的溶血率,并进行比较。【结果】新型吸引头组溶血率明显低于传统吸引头组(P < 0.01),不同出血速度下各组溶血率差异无统计学意义(P > 0.05),吸引头类型与出血速度之间的相互作用无统计学意义(P > 0.05)。 【结论】 采用新型吸引头进行自体血液回收,能有效减少负压抽吸对红细胞的破坏,降低回收血液中红细胞的溶血率,提高自体血液回收的效率;出血速度对回收血液中红细胞溶血率没有影响;不同吸引头类型与出血速度之间不存在交互作用。
Abstract: 【Objective】 To compare hemolysis in blood collected with new type suction tip and traditional suction tip under different blood velocities. To evaluate the clinical value of the new type suction tip. 【Methods】 Surgical drainage blood within 6 hours was collected after orthopedics operations. Drainage blood was randomly divided into 2 groups (each group contains 45 cases): A group (with traditional suction tip), B group (with new type suction tip). Each group was set three subgroup according to different velocities of blood flow: A1, B1(V = 2 mL/min), A2, B2 (V = 4 mL/min), A3,B3(V = 8 m L/min). A model was built using suction tip with negative pressure (-0.2 MPa). The velocity of anticoagulant was set for V = 1 mL/min. Hemolysis was measured in blood collected after 5 minutes’ suctioning. 【Results】 Hemolysis in new type suction tip group was significantly lower than that in traditional suction tip group (P < 0.01). There is no significantly difference among groups under different blood flow velocity in hemolysis (P > 0.05). There was no significantly interaction between the two factors. (P > 0.05). 【Conclusion】 Significant reduction of blood damage can be obtained by using the new type suction tip. The velocity of blood flow does not influence hemolysis in autologous blood. There is no significant interaction between the type of suction tip and the velocity of blood flow.
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