【Objective】To describe a case of a rare，novel mutation causing recurrent chorioamniotic membrane separation in a Chinese family with combined next-generation sequencing（NGS）and Sanger sequencing.【Methods】For the affected fetus，poten? tial mutation were detected by the conbinedcombined next-generation sequencing（NGS）and Sanger sequencing. And the prenatal diagnosis were identified by Sanger sequencing.【Results】A frameshifting mutation c.1389_1390delAG（inherited from mother），and a missense mutationc. 1006 G > C（inherited from mother）have been identified in the affected fetus（the second pregnancy）. The pre? natal diagnosis of the third fetus turns out to be a carrier，the mutation was inherited from father.【Conclusions】We describe a novel mutation in gene ZMPSTE24 ，which was considered with mandibuloacral dysplasia with type B，and that may be the cousecourse? causing of recurrent chorioamniotic membrane separation. This rare mutation constitutes an additional heterogeneous defect causing chorioamniotic membrane separation. And the conbinedcombined next-generation sequencing（NGS）and Sanger sequencing allows high resolution characterization of novel mutions that are not readily detected by present methods.