Abstract: 【Objective】 To establish U937 cell line stably expressing p62 gene and to investigate the biological characteristics of this cell line. 【Methods】 The recombinant MSCV-p62-IRES-GFP (MPIG) vector was constructed and packaged the virus for infecting U937 cell line. U937 cells successfully infected were subjected to fluorescence activated cell sorter (FACS) for sorting out the GFP positive cells. The expression of p62 was determined by Western blot. MTT was used to show the cell proliferation.CD11b and CD14 expression were detected by flow cytometry. 【Results】 U937 cells that infected with retrovirus expressed GFP and were subjected to FACS. After sorting

the green fluorescent rates of U937-GFP cells and U937-p62 cells were 98.5% and 87.3%

respectively. And the p62 protein expression was increased in U937-p62 cells than that in U937-GFP cells. Comparing with U937-GFP cells

the cell proliferation of U937-p62 cells was faster. Then flow cytometry showed there was no difference of the CD11b and CD14 expression between U937-GFP cells and U937-p62 cells. 【Conclusion】 The recombinant MPIG vector was constructed and p62 gene stably-expressed U937 cell line was established successfully. It was proved that overexpression of p62 promoted the viability but not the differentiation of the U937 cell.