Abstract:【Objectives】 To explore the expression and correlation of CENP-W

B23 and RAS in different pathologic grade gliomas. 【Methods】 The expression of CENP-W in high grade glioma tissues

low grade glioma tissues and adjacent brain tissues were detected by immunohistochemistry

then the relationship of CENP-W protein expression levels with the clinicopathologic feature of glioma patients were investigated. The expression levels of CENP-W

B23 and RAS were detected by real-time fluorescene quantitative PCR and Western blotting

its correlation to the pathologic grade and the correlation between the expressions of CENP-W

B23 and RAS was analyzed. After transfection with the specific small interference RNA (sirna) targeting CENP-W gene to repress CENP-W expression in U251 cells

the changes of RAS mrna and protein levels were detected by real-time fluorescene quantitative PCR and Western bloting. 【Results】 The expression levels of CENP-W

B23 and RAS in glioma tissues were significantly higher than those in normal tissues. There was a positive correlation between the three protein expression levels and the pathological grade of glioma. There was positive correlation between the expression level of CENP-W mrna

B23 mrna and RAS mrna. The expression of RAS was down-regulated when the expression of CENP-W was repressed by CENP-W sirna in U251 cells. 【Conclusions】 Both of CENP-W

B23 and RAS expressions are positively correlated with the pathological grade of glioma. CENP-W can regulate the expression of RAS.It implicated that CENP-W can be a novel target in gliomas treatment.