Abstract: 【Objective】 To screen the related long non -coding RNA(lncRNA) expression profile changes in the early stage osteogenic differentiation of human bone marrow mesenchymal stem cells (hMSC)

to analyze the affection between the changes and the early stage osteogenic differentiation of hMSC. 【Method】 hMSC with before and after 7days of osteogenic differentiation were taken as research subjects

the Agilent lncRNA chip technology was used to screen the lncRNA which has two fold differences in expression before and after the osteogenic differentiation. One of a quite different lncRNA in expression was selected

and UCSC Genome Browser

which is the nearby lncRNA protein -coding gene

was used for the following discussion. 【Results】 After 7days of osteogenic differentiation

hMSC had exhibited in having a mild osteoblast -like characteristic; the results of the Agilent RNA microarray screening showed that there were 923 lncRNA upregulated and 993 lncRNA downregulated after 7days of hMSC osteogenic differentiation; UCSC Genome Browser bioinformatics analysis showed that ENST00000585537 is associated with MAPK protein. 【Conclusion】 The expression of lncRNA is altered after 7days of hMSC osteogenic differentiation

part of the lncRNA which have large differential expression through the contact with related protein coding genes affect the early osteogenic differentiation function of hMSC.