Abstract: 【Objective】 To observe and explore the suppression of lentiviral shRNA on PTTG in GH3 and AtT20 pituitary adenomas cells and its effect on tumor cells’ proliferation and invasion. 【Methods】 A lentivirus shRNA expressing vector targeting PTTG gene was constructed and packaged.The rate of GH3 and AtT20 pituitary adenomas cells infected lentivirus shRNA vector was analyzed by fluorescence activated cell sorter(FACS). Expression level of PTTG mRNA and protein was analyzed by RT-PCR and Western blot. Cell proliferation was performed withMTT. Cell cycle was determined by flow cytometry. Transwell assay was used to determine the invasion ability of tumor cells. Affymetrix GeneChipprimeview was used. In microarray process to determine differences of gene expression profile. 【Results】Lentiviral-mediated shRNA efficiently reduced PTTG expression. PTTG knockdown impaired cell proliferation

colony formation andinvasion ability

and induced cell cycle arrest in GH3 and AtT20 cells. 【Conclusion】 PTTG gene silencing by shRNA mediated with lentiviral vector could significantly inhibit GH3 and AtT20 cells growth. It also can inhibit the proliferation and invasion of these pituitary adenomas cells. Microarray analysis revealed that multiple cancer-associated pathways and oncogenes were regulated by PTTG.