Abstract: 【Objective】 To explore the regulatory mechanism of PEDF involved in lipid metabolism to provide important basis for PEDF participate in a variety of metabolic diseases. 【Methods】 Crispr /Cas9 gene modification technology was used to construct PEDF -/ - mice

then obese mice model was induced by high fatty diet. The serum level of triglyceride

total cholesterol and free fatty acids was detected. Real -time PCR was used to analyze the expression of the lipid metabolism -related genes (ATGL

PPAR -γ

FAS

HSL). 【Results】 Genotype identification and protein level tests showed PEDF -/ - mice was successfully build. After HFD

the weight

fat deposition and serum lipid levels in wild type mice were higher than normal diet group(P < 0.05)

and the differences were statistically significant

suggesting that obesity model were successfully built. While in PEDF knock-out HFD mice

the weight

fat deposition

and the overall blood lipid levels were significantly higher than the normal diet group(P < 0.05) and the wild type HFD group(P < 0.05)

the difference is statistically significant. Real time PCR results showed that

in high fatty group of wild type mice

ATGL

PPAR-γ and HSL transcription levels are significantly lower than normal diet group of wild type mice(P < 0.05); But in PEDF knock-out mice

the transcription level of ATGL

PPAR-γ and HSL significantly lower than the wild type mice(P < 0.01)

and FAS levels increased significantly in normal diet

but significantly downregulated in high-fatty diet; In PEDF knock-out mice

high-fatty diet group of ATGL mRNA level of high-fatty diet group were higher than the normal diet group

PPAR-γ and HSL mRNA level is lower than the normal diet group. 【Conclusion】 PEDF-/- mice was successfully build. We conjectured that PEDF might involved in lipid metabolism and lipid deposition in mice by affecting the expression of lipid metabolism-related genes (ATGL

PPAR-γ

FAS

HSL) in adipose tissue.