Abstract: 【Objective】 To establish an induced pluripotent stem cell(iPSC) line from the urinary cell of a patient with Bruagada syndrome (BrS). 【Methods】 Human urinary cells infected with retrovirus mixture containing OCT4

SOX2

KLF4

and C-MYC genes were induced into ESC-like colonies. The iPSC generated were analyzed for ESC markers: colony morphology

alkaline phosphatase activity

the expression pattern of ESC-marker genes

karyotype and differentiation ability. 【Results】 The iPSC generated was similar to ESC in morphology

ESC-marker genes expression and karyotype. Embryoid body-mediated in vitro differentiation of iPSC can form all the 3 germ layers. Subcutaneous injection of iPSC into NOD-SCID mice resulted in teratomas containing tissues from all the 3 germ layers. 【Conclusion】 The BrS patient-specific iPSC cell line was successfully established. It was a serviceable model to study the pathogenesis of BrS and develop drug screening assays.