Abstract:【Objective】 To investigate the genetic polymorphism of 45 autosomal STR loci included in PowerplexTM 16

STRtyper-10F/G and AGCU 21+1 systems in Guangdong Han population and assess their value in forensic medicine. 【Methods】 Samples from 256 two generation pedigrees (including 512 unrelated individuals) were amplified with 3 systems

respectively. The amplified products were separated with ABI 3500XL genetic analyzer and analyzed with GeneMapper ID-X software. The forensic parameters were calculated. Hardy-Weinberg equilibrium of each locus and pairwise linkage disequilibrium were tested. 【Results】 After the Bonferroni correction

the genotypes of 45 STR loci were in accordance with Hardy-Weinberg equilibrium

no linkage disequilibrium was found. The average Ho of the 45 STR loci was 0.765

the average DP was 0.905 and the average PIC was 0.733. The CMP of the 45 STR loci was 9.48 × 10-50

the CPEduo was 0.999 999 999 94 and the CPEtri was 0.999 999 999 999 999 990 4. 【Conclusion】 We obtain the population data of 45 STR loci in Guangdong Han population

providing basic data for forensic personal identification and parental testing. Combining PowerplexTM 16

STRtyper-10F/G and AGCU 21+1 systems can meet the requirements in difficult cases such as mutated cases

duo paternity cases and kinship testing.