【Objective】 To investigate the effects of Trx2

GSH

and GSH-PX on acute kidney injury (AKI) induced by rat orthotopic liver transplantation (OLT). 【Methods】 Thirty-five Sprague-Dawley (SD) rats were divided into 5 groups: sham group

and other 4 groups: 4 h

8 h

16 h

and 24 h after orthotopic liver transplantation groups. Rats in sham group only suffered from the process of laparotomy and vascular separation

while in other groups all subjected to OLT. Kidney pathological changes after OLT and levels of ·OH

H2O2

MDA

LDH were observed. GSH

GSH-PX and expression of Trx2 were detected to explore their protection effects on AKI induced by OLT. 【Results】 Eight hours after OLT

kidney pathological damage was most serious. From 4 hours after OLT

levels of ·OH

H2O2

MDA

LDH increased obviously

and recovered to the normal level until 24 hours after OLT. By contrast

both GSH and GSH-PX decreased from 4 hours after OLT and came back to the normal level at 24 hours after OLT. Expression of Trx2 increased from the time point of 4 hours after OLT and reached the top at 24 hours after OLT

then

declined gradually. Correlation analysis showed that there were negative correlations between kidney damage and levels of GSH and GSH-PX (P < 0.01)

but positive correlations between kidney damage and Nrf2 expression (P < 0.05). 【Conclusion】 AKI induced by OLT changed dynamically. Eight hours after OLT

AKI was most obvious and then

recovered gradually. Kidney damage was relative with GSH and GSH-PX decrease. Increase of Trx2 expression could protect against AKI induced by OLT.