Abstract: 【Objective】 To analyze the status of DNA methylation in early nasopharyngeal carcinoma

and to find out the DNA methylation profile of early nasopharyngeal carcinoma preliminarily. 【Methods】 Four pairs of paraffin specimens of NPC(T1N0M0) and inflammatory nasopharyngeal tissues were collected to extracted DNA; The enriched genomic methylation fragments of two group specimens were used for high-throughput sequencing analysis by NimbleGen Human DNA Methylation 3 × 720K CpG Island Plus RefSeq Promoter Array. We scanned the hybridization signal

processed preliminary data and analyzed the genomic DNA methylation differences between early stage NPC and matched normal nasopharyngeal tissues. 【Results】 The number of methylated genes in all four cases of NPC paraffin specimens are 1134

and the number of methylated genes in the inflammatory nasopharyngeal tissues are 2260. Among the early stage NPC and compared specimens

the number of differentiation methylated genes are 218

and they might be the DNA methylation profiles of early stage NPC patients.In addition

the number of methylated genes in the four inflammatory nasopharyngeal tissues but not in the NPC paraffin specimens is 1558. 【Conclusion】 In this study

the DNA methylation profiles of early stage NPC were initially built. It laid a foundation for the further development of more precise profiles

meanwhile

it also provides possibility for looking for new molecular markers of NPC.