Abstract:【Objective】 To establish a zebrafish xenotransplantation model of cervical cancer and investigate the pro -angiogenic effects of cervical cancer.【Methods】 A linearized plasmid pcDNA3.0 vector expressing Ds -Red fluorescent protein were used to fluorescently label human cervical cancer SiHa cells. The fluorescent -labeled SiHa cells were then transplanted into the FLK 48 hpf transgenic zebrafish embryos using microinjection. The survival

proliferation and induction of angiogenesis were evaluated in zebrafish xenotransplantation embryos and larvae. 【Results】 Different numbers of xenograft human cervical cancer SiHa cells had different effects on zebrafish and transplantation of 50 -100 c SiHa cells achieved an excellent angiogenesis. Xenograft SiHa cells significantly increased new blood vessels compared with the control group. The numbers of the vertical branch of subintestinal vein outside yolk in matrigel control group and xenograft group were 2.8 ± 1.4% and 8.0 ± 2.7% in day 1

5.4 ± 2.2% and 7.4 ± 3.2% in day 2 after injection (all P < 0.01). 【Conclusion】 This study has successfully established zebrafish xenotransplantation model of cervical cancer and demonstrated the pro -angiogenesis of cervical cancer

which provides a new technology platform for the study of the pathogenesis of cervical cancer development of new compounds and gene targets for anti -tumor angiogenesis.