Abstract:【Objective】 To explore the clinical value of endoscopic ultrasonography combined with the expression of matrix metalloproteinases-7(MMP-7) and matrix metalloproteinases-9(MMP-9) in serum in preoperative staging of gastric carcinoma. 【Methods】 500 patients with gastric carcinoma were diagnosed by electric gastroscopy and EUS. The preoperative staging of gastric carcinoma was measured by EUS and compared by pathologic staging and the expression of MMP-7

MMP-9 in serum. The serum levels of MMP-7 and MMP-9 in 500 patients with gastric carcinoma were measured with the methods of ELISA (enzyme-linked immunosorbent assay). 【Results】 The sensitivity (or specificity) of EUS in estimating invasive depth of gastric carcinoma were T1 93.7% (92.2%)

T2 67.8% (65.6%)

T3 78.4% (80.6%) and T4 91.5% (90.5%)

while the total accuracy rates of T stage by EUS were 80.2%. The sensitivity (or specificity) of EUS in estimating lymphatic metastasis of gastric carcinoma were N0 89.4% (80.8%)

N1 63.8 %(74.1%)

N2 88.7% (92.6%) and N3 82.8 %(73.9%)

while the total accuracy rate of N stage by EUS was 83.0%. The expression levels of MMP-7 and MMP-9 in serum were closely correlative with invasive degree

lymphatic metastasis

pathologic staging in gastric carcinoma (P < 0.05). The sensitivity(or specificity) of combining EUS and MMP-7

MMP-9 in estimating invasive depth of gastric carcinoma were T1 93.7% (92.2%)

T2 88.4% (93.9%)

T3 94.6%(92.5%) and T4 91.5%( 90.5%)

while the total accuracy rate of T stage by EUS combined with the expression of MMP-7 and MMP-9 was 92.4 %. The sensitivity(or specificity) of combining EUS and MMP-7

MMP-9 in estimating lymphatic metastasis of gastric carcinoma were N0 97.9% (97.9%)

N1 88.3% (93.3%)

N2 95.3%(94.4%) and N3 92.9% (90.2%)

while the total accuracy rate of N stage by EUS combined with the expression of MMP-7 and MMP-9 was 94.0%. The total accuracy rate of combining EUS and MMP-7

MMP-9 in estimating invasive depth (or lymphatic metastasis) of gastric carcinoma was significantly higher than that of EUS. 【Conclusions】 MMP-7 and MMP-9 serum levels are closely correlated to pathologic staging of gastric carcinoma

so combining EUS and MMP-7

MMP-9 can increase the accuracy rate to decide the invasion and metastasis in the patients with gastric carcinoma.