Abstract:【Objective】To research the possible effects of Axl deglycosylation on the proliferation and invasion of murine hepatocellular carcinoma cell line Hca-F.【Methods】 Western blotting was used to analyze the expression profile of Axl glycoprotein in mouse hepatocellular carcinoma cell line Hca-F treated with tunicamycin. MTT assay and ECM invasion assay were utilized to evaluate the effect of Axl deglycosylation on the Hca-F cell proliferation and invasion. 【Results】 By treatment with tunicamycin

the expression level of Axl glycoprotein was markedly changed. The new bands that appeared were consistent with the size of the core protein

which were the result of tunicamycin inhibiting Axl glycosylation. The Hca-F cells proliferation and invasion capability decreased

comparing with control (P < 0.05). 【Conclusion】 Axl deglycosylation inhibits tumor cells proliferation and invasion in murine hepatocellular carcinoma cell line Hca-F.