Abstract: 【Ojective】 The current study was designed to determine whether 17β-E2 decrease Th17 cell migration by inhibiting MHC II expression and cytokines secretion (focus on IL-17 and TGF-β) in astrocytes. 【Methods】 Primary astrocyte cultures were treated as follows: untreated

treat with IFN-γ (20 ng/mL) alone or in combination with 0.28 ng/mL

2.75 ng/mL

27.50 ng/mL 17β-E2 for 36 h. ERα and ERβ expression in astrocytes with different treatment were detected by western blotting. MHC II expression were analyzed by immunofluorescence and flow cytometry. Co-stimulatroy molecules expression were analysed by flow cytometry. IL-17 and TGF-β secretion were examined by ELISA. Th17 cells migration was detected by transwell assay. 【Results】 IFN-γ induce the expression of ERα

but not ERβ. 17β-E2 treatment at different concentrations from 0.28 ng/mL to 27.50 ng/mL significantly decreased MHC Ⅱ and co-stimulatory molecules expression in IFN-γ activated astrocytes. 17β-E2 at 27.50 ng/mL significantly inhibited IL-17 production in IFN-γactivated astrocytes

but had no effect on TGF- β secretion. 17β-E2 at 2.75ng/mL decreased Th17 cells migration by IFN-γ activated astrocytes. 【Conclusions】 These data demonstrate that 17β-E2 decreases Th17 cells migration partially via inhibiting MHC Ⅱ expression and IL-17 secretion in IFN-γ activated astrocytes.