Abstract: 【Objective】 To study the anti-inflammatory and analgesic activities of bufalin in vivo. 【Methods】 Carrageenan-induced paw edema assessed the anti-inflammatory activity of this compound. Western blot detected the NF-κB signaling during this effect. The anti-nociceptive activities were evaluated with acetic acid-induced writhing test and formalin test. 【Results】 Our data showed that bufalin (0.3

0.6 mg/kg

i.p.) potently decreased carrageenan-induced paw edema. Bufalin down-regulated the expression level of nitric oxide synthase (iNOS)

cyclooxygenase-2 (COX-2)

and tumor necrosis factor-?琢 (TNF-?琢) during this effect. Further studies demonstrated that bufalin significantly inhibited the nuclear translocation of NF-κB p65. Bufalin also reduced acetic acid-induced writhing and the licking time in formalin test. 【Conclusion】 Bufalin presented in vivo anti-inflammatory and analgesic effects

probably through the inhibition of NF-κB activation.