【Objective】 To investigate the effect of angiotensin II (AngII) on the expression and the translocation of Glucose Transporter-1 (GLUT-1) in cultured mouse podocyte cells. 【Methods】 Method one

The expression of mRNA and protein of GLUT1: Cultured mouse podocyte cells in vitro were randomly divided into normal control group(C)

AngII at the concentration of 10-10 mol/L (low concentration group)

10-8 mol/L (middle concentration group)

10-6 mol/L (high concentration group). The expression of mRNA and protein of GLUT1 were detected by semi-quantitative RT-PCR and Western blot respectively. Method two

the distribution of GLUT1 in the plasma membrane (translocation) of podocyte: Podocyte Cells were divided into normal control group (C)

insulin 10-6 mol/L

AngII 10-6 mol /L plus insulin 10-6 mol/L and losartan (LO) 10-6 mol/L plus AngII 10-6 mol/L and insulin 10-6 mol/L groups. The way of Western blot and immunocytofluorescence were used. 【Results】 Compared to control group

AngII at the concentration of low

middle

and high groups increased GLUT1 transcription by 5.89% (P 0.05)

and by 11.33% in the LO 10-6 mol/L plus AngII 10-6 mol /L and insulin 10-6 mol/L group (P < 0.01). Immunocytofluorescence indicated the expression of GLUT1 in the plasma membrane of insulin group was the strongest in the four groups and losartan could increase the GLUT1 expression in the plasma membrane partly. 【Conclusion】 AngII increases the expression of GLUT1

which is concentration dependent. Ang II prohibits the insulin-induced increasing of GLUT1 in the plasma membrane (translocation)

and losartan could partly blocks the effect of AngII on the function of insulin.