【Objective】 To explore protective effect of different patterns to form local isolation subcutaneously which prevent rabbits skin defending thermal injury during Radiofrequency ablation (RFA) process of superficial lymph nodes. 【Methods】 21 New Zealand white rabbits established reactive hyperplasic lymph nodes in bilateral popliteal fossa

were divided into 3 groups (n = 7): Group A

performed RFA without injected physiological saline solution subcutaneously to form local isolation. Group B

injected 5 mL physiological saline solution subcutaneously near lymph node before RFA: Group C

injected 1 mL physiological saline solution subcutaneously near lymph node before RFA

and followed by continuous injection of physiological saline solution using syringe pump with 1 mL/min during RFA process. Contrast-enhanced ultrasonography (CEUS) was performed after RFA and the area of lesions were measured; skin injury was observed in 1 hour and 1 week later after RFA. 【Results】 CEUS showed the RFA lesions of A group was larger (P < 0.01). For the skin change of 1 hour and 1 week later

A group showed the most serious injury (P < 0.01)

while B group and C group appeared slighter injury. 【Conclusions】 Subcutaneous injection of physiological saline solution to form local isolation before RFA in the superficial lymph nodes can protect the skin effectively

and the effect of continuous injection will be more helpful.