Abstract: 【Objectve】To screen childhood acute lymphoblastic leukemia (ALL) prognostic-related microRNAs (miRNA) and biomarker

provide experimental evidence for further stratified therapy in childhood ALL. 【Methods】 miRNA expression profile was analyzed within 10 diagnosis-complete remission (CR) childhood ALL bone marrow samples (as gene chip group). Abnormal expression of miRNA was verified by qRT-PCR (as experimental group). The correlation of abnormal miRNA with prognosis of ALL was analyzed through clinical data. 【Results】 For samples at diagnosis

the miR-708

miR-181b

miR-199a-3p

miR-320a

miR-145 possessed a higher level of miRNA expression. In addition

lower expression of miRNA was detected in miR-223

miR-1244

miR-494

miR-124

miR-23a

miR-27a

miR-181a*

and miR-23b. In validation group

the miR-708

miR-223 and miR-27a were selected for further verify by qRT-PCR

the result was consistent with experimental group. Relapse-free survival analysis showed that patients with higher miR-708

miR-223 or miR-27a expression at diagnosis had a higher RFS rate compared with the lower expression group. 【Conclusion】 miRNA was strong biomarkers for predicting the outcome of ALL. High miR-708

miR-223 and miR-27a expression levels in newly diagnosed childhood ALL had a better outcome compared with the lower expression patients. suggesting the potential application value in guiding clinical stratified treatment.