【Objective 】 To investigate the effects of advanced glycation end products (AGE) on the expression of angiopoietinlike protein 4 and its mechanisms.【Methods 】 Endothelial cells were incubated with various concentrations of AGE for 24 hours

the expression of angiopoietinlike protein 4 were detected by realtime PCR and Western blot analysis

the concentration of angiotensin Ⅱin conditioned media and cell lysates were measured by enzymelinked immunosorbent assay

FITClabeled dextran filtration assay and transendothelial electrical resistance were performed to evaluate endothelial permeability. 【Results】 AGE increased the expression of angiopoietinlike protein 4 parallel with an increase in the levels of angiotensin Ⅱ(P < 0.05). Incubation with AGE also resulted in a significant increase in endothelial permeability (P < 0.05). However

pretreatment with angiotensin Ⅱreceptor blocker Losartan(10-5 mol/L) blunted these effects induced by AGE (P < 0.05). 【Conclusions】 AGE upregulated the expression of angiopoietinlike protein 4 via activation local reninangiotensin system in endothelial cells

which may be a new mechanism for AGEs increasing endothelial permeability.