【Objective】To evaluate the effects of SPIO labeling on the IRP1 gene and protein expression in osteoblastlike differentiated cells. 【Methods】 To label the RADSC with SPIO and transfection agent PLL

then induce SPIO labeled and unlabeled RADSC into osteoblastic differentiation cells. Realtime quantitative PCR and Western blot was used to exam the expression level of IRP1 gene and protein on the 0 day

7 day

14 day

and 21day after induction. 【Result】 The expression of IRP1 gene of osteoblastlike cells had not changed significant after labeling (F = 1.571

P = 0.239). The expression of IRP1 protein of osteoblastlike cells had not changed significant after labeling (F = 0.547

P = 0.500). 【Conclusion】 OsteoblastLike cells were not effected by SPIO not only in the aspect of IRP1 gene

but also in IRP1 protein.