【Objective】 To investigate the expression of cytokines in T cells and NK cells from human peripheral blood mononuclear cells in response to dengue virus in vitro

and to explore the immune response after dengue virus infection. 【Methods】 Flow cytometry was used to detect the phenotype and cytokines of T cells and NK cells from PBMC after DEN-2 infection. 【Results】 T cells express CD69

IFN-γ

TNF-α

perforin

and NK cells express CD69

IFN-γ

perforin 18 hours after DEN-2 infection. The numbers of IFN-γ and perforin expressing T cells and perforin expressing NK cells have a positive correlation with dengue virus dose. The numbers of perforin and IL-4 expressing T cells and perforin expressing NK cells increase

whereas the numbers of IFN-γ expressing T cells and NK cells decrease follow infection times. T cells expressing perforin are mainly CD8+T cells. 【Conclusion】 With in vitro infection by dengue virus

T cells can express CD69

IFN-γ

TNF-α

perforin and IL-4. And NK cells can express IFN-γ and perforin at the same time. We can conclude that T and NK cells may play an important role in the anti-dengue virus infection or immunopathology.