【Objective】 The aim of the study was to investigate the fractionated-dose irradiated Hela cells on the expression of cancer stem cell related proteins and the ability of in-vivo tumorigenicity

and to explore the feasibility of enriching cervical cancer stem cells by this method. 【Methods】 Hela cells were treated with different fractionated rays of dose 2

4

6

8

10

and 12 Gy

cumulatively 24-42 Gy. The expressing rates of ABCG2

CD44

and CD133 in irradiation groups and control group were detected by flow cytometry. Cells in each group were inoculated subcutaneously into nude mice to test their tumorigenicity in vivo.【Results】 The expression of ABCG2 was significantly increased in irradiation groups

which was the highest in Hela-R2 group [(46.89 ± 1.20)%] and much more increased with more frequencies of radiation (P < 0.05). There was no significant trend in the expression of CD44 and CD133 among each group. Cells in Hela-R2 group (4 Gy × 10) exhibited strongest tumorigenicity in vivo

followed by Hela-R5 group(10 Gy × 3).【Conclusion】 Fractionated-dose irradiation may enrich cervical cancer stem cells in vitro

with the best effect in dose 4 Gy group. Increased expression of ABCG2 and tumorigenicity in nude mice could be induced in Hela cells by this way.