【Objective】 To investigate the proteomics change in lung tissue after one-lung ventilation (OLV) induced lung injury in rabbits. 【Methods】 Twelve New Zealand white rabbits were randomly divided into two groups: two-lung ventilation (TLV) group (n = 6) and one-lung ventilation (OLV) group (n = 6). TLV group received two-lung ventilation

while OLV group received right lung ventilation. Oxygenation index (OI) was monitored. Right and left lung tissues were sampled respectively for histological analyses after surgery. Total proteins extracted from the left lung tissue of both groups were separated by 2 dimensional-difference in gel electrophoresis. Differentially expressed proteins were searched using DeCyder soft ware

then analyzed and identified with mass spectrometry. 【Results】 Oxygenation index in OLV group was less than 300mmHg after 3h of OLV and significant lower than that in TLV group (P < 0.05). The right and left lung damage scores were significantly higher than those in TLV group (P < 0.05). Between OLV group and TLV group

6 differential proteins were detected and identified

including ATP synthases

actin and peroxiredoxins

etc. 【Conclusion】 The rabbit model of OLV-induced lung injury can be successfully established. OLV-induced lung injury can lead to the change of proteom in lung tissue

and the differentially expressed proteins are mainly related to energy metabolism

cell skeleton and oxygen stress.