【Objective】 The aim of this study was to explore the effects of Coriolus versicolor polysaccharide-B (CVPs-B) on the metastatic characteristics of human esophageal carcinoma cell line Eca109 in vitro. 【Methods】 The expression of CXCL12 in the cell line Eca109 was detected by Western blot. The adhesive ability of Eca109 cells to extracellular matrix matrigel was evaluated by MTT assay. The invasive ability of Eca109 cells was measured by Transwell experiment. The locomotion ability of Eca109 cells was measured by scrape assay.【Results】 Compared with control group

CXCL12 protein expression in experimental group was down-regulated (P < 0.05). Meanwhile

the number of Eca109 cells infiltrated Transwell membrane in experimental group was decreased (P < 0.05). The adhesion rate of experimental group cells was significantly reduced (P < 0.05). The locomotion ability of experimental group cells was reduced. 【Conclusion】CVPs-B can reduced expression of CXCL12 protein in esophageal carcinoma cells. It can inhibit metastatic potential of Eca109 cells. CVPs-B maybe inhibit the transfer characteristics of squamous cell carcinoma of the esophagus through the CXCL12/CXCR4 pathway.