【Objective】 To investigate the expression profile of miRNAs in the prostate cancer and benign prostatic tissues

and to evaluate the role of differential miRNAs in the regulation of oxidative signaling of prostate cancer. 【Methods】 The total RNA was extracted from prostate cancer tissues and adjacent non-tumorous tissues (100 mg each) and the expression of miRNAs were detected by miRNA PCR Array (Denmark

Exiqon). Prostate cancer cell line PC-3 was incubated with hydrogen peroxide (H2O2

250 μmol/L) for 4 hours

and then was cultured in normal condition for 12 hours. The intracellular reactive oxygen species (ROS) and the expression of miR-96 were detected at indicated time points. 【Results】 Compared to the normal prostate tissues

miR-144

and miR-216a were up-regulated 5.9 times

and miR-96 was up-regulated 30.4 times in the prostate cancer tissues

while miR-488 and miR-873 were down-regulated to 4.9%(P<0.05). The intracellular ROS of PC-3 cells was as 5.2 times as to that in the RWPE-1 cells (P < 0.05)

and the expression of miR-96 in PC-3 was 15.4 times compared to that in the RWPE-1 cells (P < 0.05). In the 1 h and 4 h group

H2O2 induced 4.3 and 6.4 times of ROS compared to that in the control (P < 0.05)

with 10.2 and 18.9 times of miR-96 expression compared to that in the control (P < 0.05). In the 10 h and 16 h group

the intracellular ROS remained 2.5 and 1.2 times compared to the control (P > 0.05)

with 2.7 and 1.9 times of miR-96 expression compared to the control (P > 0.05). 【Conclusion】 This study had identified several different expression miRNAs in prostate cancer. miR-96 plays an important role in the oxidative signaling of prostate cancer. It may be developed as an important molecular target for the treatment of prostate cancer.