【Objective】 To resolve the difficulty in blood group typing test and antibody identification test of autoimmune hemolytic anemia (AIHA) with both warm and cold autoantibodies. 【Methods】 Patient′s RBC was used for ABO and rhesus typing test after it had been eluted with gently heat and chloroquine. The ABO reverse typing test was performed after the autoantibodies in patient′s plasma had been absorbed with O group blood cell. Polyethylene glycol (PEG) adsorption method and auto-adsorption method were studied by comparing the total time and numbers of the adsorption. Antibodies were identified with ethylether elute. 【Results】 ABO and Rh blood group were typed correctly in 21 and 15 cases

respectively. Seven cases were detected to have both allo-antibody and auo-antibody

including anti-S

anti-Ce

anti-c and anti-E

however

antibodies specificity had not been identified in 3 cases. Fourteen cases had only autoantibody. The mean time required by PEG adsorption and auto-adsorption for autoantibody removal was 39.3 minutes (total

825 minutes) and 98.7 minutes (total

2 070 minutes). The mean number of autoadsorption for removing autoantibody were 2.6 (total

55) and 3.1 (total

66). 【Conclusion】 Gently heat elute combines chloroquine elute is a good choice to perform blood group typing test for AIHA patients with both cold and warm autoantibodies. PEG method is a rapid

simple and effective way to remove autoantibody and detect underlying alloantibody

which can be used in routine practice.