【Abstract】 Objective To dynamically detect the bacterial distribution and drug sensitivity of the secretion of chronic supperative otitis media

and to get the message of pathogens trends and clinical medication of chronic otitis media. Methods Tympanic pus were collected and cultured from hospitalized patients in the last three years with chronic suppurative otitis media

the data of pathogenic bacteria，drug sensitivity，minimum inhibitory concentration and the number of corresponding inhibited strains were statistics and compared with the data from 2002-2005. Results (1) There were 254 strains of microbes isolated from 369 ears. 23 of them were fungi

231 were bacteria. Coagulase-negative staphylococci( in 71 of 231 ears

30.74%)

Pseudomonas aeruginosa (in 53 of 231 ears

22.94%)

Staphylococcus aureus (in 44 of 231 ears

19.05%)

Proteus mirabilis (in 12 of 231 ears

5.19%)were the most frequently insolated pathogens. (2) The cholesteatoma otitis media were mainly infected by Proteus mirabilis and Pseudomonas aeruginosa

and the non-cholesteatoma otitis media were mainly infected by Coagulase-negative staphylococci. The level of bone destruction might be related to pathogenic bacteria. Pseudomonas aeruginosa caused moderate or severe bone destruction

and Coagulase-negative staphylococci caused relatively mild bone destruction. (3)Pseudomonas aeruginosa were sensitived to piperacillin / tazobactam

ceftazidime

cefepime

ciprofloxacin

gentamicin

tobramycin

and imipenem; Proteus mirabilis were sensitive to all experimental drugs; Coagulase-negative staphylococcus and Staphylococcus aureus were sensitived to quinoline Nupu Ting / dalfopristin

teicoplanin

vancomycin

moxifloxacin

gentamicin

ciprofloxacin. ciprofloxacin and moxifloxacin could inhibit more than 90% bacteria when their concentration were 0.5 mcg/ml. (4)Compared with the drug sensitive trail of four common pathogens during 2002-2005

the result were similar. Conclusion Coagulase-negative staphylococci

Pseudomonas aeruginosa

Staphylococcus aureus and Proteus mirabilis are the main pathogenic bacteria of chonric supperative otitis media

and these four bacterias have their own characteristics in clinical classification and bone destruction of chonric supperative otitis media. Sensitivity test plays a guiding role in clinical treatment.