【Objective】To evaluate the influence of hepatocyte transplantation by different pathways on liver perfusion of rats with cirrhosis using CT perfusion imaging scans to select safe

reliable and effective means of transplantation. 【Methods】Fifty rats of the hepatic cirrhosis model were randomly divided into 5 groups. The first 4 groups (G1

G2

G3

and G4) were transplanted 2 mL epatocytes suspension (cell number of 2 × 106) through intra-hepatic artery (G1)

portal vein (G2)

splenic artery (G3)

and tail vein (G4)

respectively. The control group (G5) were not intervened with at all. Liver CT perfusion imaging scans were performed in the rats of each group before 1 week and 4

8 weeks after transplantation. The amount of hepatic artery perfusion (HAP)

portal vein perfusion (PVP)

total hepatic perfusion (THBP) was calculated and analyzed statistically. 【Results】 Four weeks after transplantation

the differences of HAP levels in G1 group

G2 group versus G3

G4

G5 groups were statistically significant (P <0.05). Eight weeks after transplantation

the differences of HAP levels among 5 groups were not statistically significant (P > 0.05). Four and 8 weeks after transplantation

there was no statistically significance in the variation of PVP levels in G1 group

G2 group versus G3

G4

G5 difference (P > 0.05). 【Conclusion】 The results show that hepatocytes transplantation by different pathways can not be effective in improving liver perfusion. On the contrary

liver transplantation by hepatic artery and portal vein increased the risk of disorders.