【Objective】In the high-fat-induced insulin resistance rat model

study the role and mechanism of caveolin-3(CAV-3) in the effect of resveratrol(RSV) on insulin resistance in skeletal muscles. 【Methods】Rats were divided into three groups: normal diet group (NORM)

high-fat diet group (HFD)

high-fat diet plus resveratrol group (HFD + RSV). Study the protective effect of resveratrol on insulin resistance by intraperitoneal glucose tolerance test

fasting glucose

fasting serum insulin

and 2-deoxy-[3H] glucose uptake in vitro soleus muscles. Test the role of caveolin-3 (CAV-3) and glucose transporter 4 (GLUT4) in the above processes through Western blot. 【Results】RSV has protective effect on high-fat diet-induced insulin resistance. RSV elevated insulin-stimulated glucose uptake in isolated soleus muscle in vitro by enhancing GLUT4 translocation to the plasma membrane rather than increasing GLUT4 protein expression. RSV increased CAV-3 protein expression

which contributed to GLUT4 translocation.【Conclusion】The results showed that RSV ameliorates insulin resistance by increasing glucose uptake in skeletal muscles through a CAV-3-dependent pathway

and the mechanism was that RSV up regulates CAV-3 protein expression and enhances GLUT4 translocation to plasma membrane.