【Objective】 To investigate the influence of ultrasound insonation to the shape and movement of Sonazoid microbubbles outside and inside of Kupffer cells. 【Methods】 Kupffer cells were isolated from rats. Phagocytosis of Sonazoid microbubbles were observed under inverted phase contrast microscope. Ultrasound insonation was performed using a clinical ultrasound scanner

phenomena of microbubbles outside and inside of Kupffer cells in an acoustic field with different acoustic pressure were observed.【Results】The phagocytosis rate of Sonazoid microbubbles by Kupffer cells was 91%. The thresholds in term of mechanical index (MI) for free microbubbles to begin oscillation

reach best oscillation

and destruction were 0.21

0.44

and 1.03

respectively. The thresholds of destruction were 1.18 for adherent microbubbles

and 1.37 for phagocytosed microbubbles

respectively. 【Conclusions】 Sonazoid microbubbles could be phagocytosed by Kupffer cells in vitro. Acoustic pressure of ultrasound insonation affects the movement and the shape of Sonazoid microbubbles outside and inside of Kupffer cells obviously.