Abstract : 【Objective】 To observe the in vitro antitumour effects of epirubicin-nanogold compounds(EPI-AuNP). 【Methods】 EPI-AuNP was prepared by chemosynthesis and investigated using UV-Vis spectrophotometer

fluorescence studies

dynamic light scattering and zeta potential. Human umbilical vein endothelial cells (HUVECs) and HepG2 cells were divided into 4 groups: AuNP treatment group

EPI treatment group

EPI-AuNP treatment group and control group. After seeded in 96-well plate and cultured for 24h separately

HUVECs and HepG2 cells were treated with 200?l of AuNP

EPI

EPI-AuNP and serum-free medium respectively. Inhibition effect of each group on the HUVECs and HepG2 cells was assessed using MTT colorimetric method. UV-Vis spectrophotometer was applied to detect the cells epirubicin accumulation of different groups. 【Results】 A red shift in the SPR band maxima in the EPI-AuNP spectrum (λmax?525 nm) as compared with the spectrum of AuNP alone(λmax?520 nm) ; The fluorescence intensity of EPI (100mg /L) was (195.2±7.5) and EPI-AuNP was (16.4± 5.0)

P=0.000; The hydrodynamic diameter of AuNP was (14.34?0.75) nm while EPI-AuNP was (18.54?1.84) nm; meanwhile

the zeta potential of AuNP was (-21.19?0.64) mV while EPI- AuNP was (-15.34?0.72)mV

P<0.01. The HUVECs survival rate of EPI-AuNP treatment group [(21.29 ?1.51)%] was lower than the EPI group[(29.25?1.59)%]; The HepG2 cells survival rate of EPI-AuNP treatment group[(43.82?2.21)%] was lower than the EPI group[(71.10?4.16)%]

P<0.01. 【Conclusions】 EPI-AuNP has been synthesized and indicated enhanced drug potency in vitro by MTT assay.