【Objective】 To study the content change of TNF-α

IL-1β

MMP-3

MMP-9

and TIMP-1 in the rats after cerebral ischemia and reperfusion. To clarify the role of TNF-α and IL-1β in brain damage with ischemia rat caused by MMP and TIMP-1. 【Methods】 The 56 male SD rats were randomly divided into sham-operation group and ischemia 2 h reperfusion 6 h

12 h

24 h

48 h

72 h

7 d group. The local cerebral ischemia reperfusion model was established by intraluminal thread occlusion of the middle cerebral arteries occlusion (MCAO)

the level of TNF-α

IL-1β

MMP-3

MMP-9

and TIMP-1 were determined by enzyme-linked immunosorbent assay (ELISA). 【Results】 Compared with sham-operation group

the content of TNF-α and IL-1β in brain tissue and blood were significantly higher in reperfusion 6 h (4.38 ± 0.73 vs 2.63 ± 0.14

5.28 ± 0.71 vs 3.46 ± 0.47; 22.34 ± 3.56 vs 12.13 ± 4.26

9.56 ± 0.85 vs 4.23 ± 0.83; P < 0.05)

and reached the peak at the 12th hour after reperfusion

and then fell down. MMP3 and MMP-9 content increased at the 6th hour after reperfusion. Till the 48th hour it reached the peak

and then fell down. The content of TIMP-1 in brain tissue were significantly lower in reperfusion group than that in the sham-operation group. And the content of TNF-α and IL-1β were positively correlated with MMP-3 and MMP-9 (P < 0.05)

negatively correlated with TIMP-1. 【Conclusion】 MMP-3 and MMP-9 play an important role in cerebral ischemia-reperfusion injury. Inflammatory factor can increases the production of MMP-3 and MMP-9 in brain tissue

decrease the production of TIMP-1

and further promote the brain damage cause by ischemia reperfusion.