【Objective】 To investigate the changes of pathogens and drug sensitivity of pathogenic bacteria of neonatal ventilator-associated pneumonia (VAP). 【Methods】 Retrospective analysis was performed on the clinical data of 58 mechanical ventilated (≥48 h) patients who were diagnosed VAP in NICU from January 2004 to January 2012. And they were divided into two groups according to the time of disease occurrence to study the changes of isolated pathogens and drug sensitivity of pathogenic bacteria. 【Results】 38 strains of pathogens were isolated from 26 patients with VAP from January 2004 to January 2008

the main pathogenic bacteria include: 8 strains of Stenotrophomonas maltophilia

5 strains of Staphylococcus haemolyticus

4 strains of Pseudomonas aeruginosa

4 strains of Enteroloccus faecium

3 strains of Enterobacter cloacae. 42 strains of pathogens were isolated from 32 patients with VAP from January 2008 to January 2012

the main pathogenic bacteria include: 7 strains of Staphylococcus epidermidis

5 strains of Klebsiell

4 strains of Staphylococcus haemolyticus

4 strains of Acinetobacter baumannii

4 strains of Enteroloccus faecium. Stenotrophomonas maltophilia were partially sensitive to Levofloxacin and Ticacillin/ca. Other gram-negative bacillus were sensitive to Carbopenem antibiotics

Levofloxacin and Amikacin; Gram-positive bacillus were all sensitive to Vancomycin

Tiecoplanin

and Linezolid. 【conclusion 】 The pathogenic bacteria has severe drug resistance. The main isolated pathogens are usually multi-drug resistance opportunistic bacteria. The spectrum of pathogenic bacteria was significantly changed in the past eight year.