胚胎干细胞分化为表皮样细胞的基因表达谱差异研究

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胚胎干细胞分化为表皮样细胞的基因表达谱差异研究

更新时间：2023-12-12

- 胚胎干细胞分化为表皮样细胞的基因表达谱差异研究
- Genome-wide expression profiles analysis of ES cells and ES derived epidermal-like cells
- 中山大学学报（医学科学版） 2012年33卷第2期
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  中图分类号： R329.2+8
- 网络首发：2012-03-20，
  
  纸质出版：2012
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胚胎干细胞分化为表皮样细胞的基因表达谱差异研究[J]. 中山大学学报（医学科学版）, 2012,33(2).

Genome-wide expression profiles analysis of ES cells and ES derived epidermal-like cells[J]. Journal of Sun Yat-sen University (Medical Sciences), 2012, 33(2).
胚胎干细胞分化为表皮样细胞的基因表达谱差异研究[J]. 中山大学学报（医学科学版）, 2012,33(2). DOI：

Genome-wide expression profiles analysis of ES cells and ES derived epidermal-like cells[J]. Journal of Sun Yat-sen University (Medical Sciences), 2012, 33(2). DOI：

摘要

[摘要] 目的：用全基因表达谱芯片技术筛选小鼠胚胎干细胞（ESC）定向分化为表皮样细胞(Epidermal-like cells

ELC)的差异表达的基因并对其进行分析，以进一步阐明ESC分化为ELC的分子机制。方法：利用人羊膜建立体外诱导体系，将小鼠ESC诱导定向分化为ELC，做3次生物学重复，分别取未分化的ESC和诱导分化的ELC提取总RNA进行扩增、Cy3标记，与NimbleGen 135k 小鼠全基因表达谱芯片（含44170个基因）杂交，荧光扫描，筛选出差异表达基因，并进行GO分析和pathway分析，用实时定量PCR对结果进行验证。结果：芯片筛选结果显示，分化后的ELC与ESC间的差异表达的基因多达4856个（Cut off：2）。基因本体（Gene Ontology，GO)分析显示与生物学过程（Biological Process

BP）相关的差异表达基因最多的是细胞过程（cellular process，CP），1931个；与亚细胞组分（Cellular Component

CC）相关的差基因中最多的是细胞和细胞组分（Cell

Cell part）相关基因，2391个；与分子功能（Molecular Function

MF）相关的差异基因中最多是的结合功能(binding)基因，1869个。Pathway分析显示，与DNA复制（DNA replication）通路相关的差异基因有23个；与蛋白酶体（Proteasome）通路相关的24个；剪接体（spliceosome）通路相关有47个；细胞周期（Cell cycle）相关的有44个。结论：ESC体外诱导定向分化为ELC过程中，基因表达谱发生了巨大的变化，提示细胞分化是一个众多基因参与的复杂的生物学过程，这些基因在分化过程所起的作用和作用机制尚需大量的实验研究阐明。

Abstract

Genome-wide expression profiles analysis of ES cells and ES derived epidermal-like cells Zhang Ren-li1△

Meng Jin-xiu2*

Liu Cai-xia1

Wen An-min1 (1 Reproductive Medicine Center of Guangdong General Hospital

2Medical Research Center of Guangdong General Hospital

Guangzhou

Guangdong

215006

China) [ ABSTRACT] AIM: Screening and analyze the whole genomic expression profile between ES derived epidermal-like cells (ELC) and undifferentiated ES cells (ESC) with chips

to explore the molecular mechanisms directed ESC differentiate into ELC. METHODS: Induced ESC to differentiate intoELC with Human amnion

and biologically repeat 3 times

extracted RNA was amplified

Cy3 labeled

and hybridized on NimbleGen 135k mouse whole gene chip (containing 44

170 genes)

get images by fluorescence scanning

screening differentially expressed genes

and perform GO and pathway analysis. Finally

use real-time quantitative PCR to validate the chip results. Results: 4856 genes show to differently expressed in ESC and ELC (Cut off: 2). GO analysis shows that in the Biological Process (BP)

the cellular process has the most differently expressed genes

including 1931 genes

in the Cellular Component (CC)

the Cell/Cell part has the most differently expressed genes

including 2391 genes; in the Molecular Function (MF)

the binding function has 1869 differently expressed genes. The Pathway analysis shows that DNA replication has 23 differently expressed genes; the Proteasome pathway has 24 differently expressed genes; the spliceosome pathway has 47 differently expressed genes; the Cell cycle pathway has 44 differently expressed genes. CONCULUSIONS: The dramatically changed expression profile in ESC and ELC indicated that

large variety of genes were involved in this complex cellular differentiation process

and how these gene worked and co worked to regulate the differentiation procedure need further investigations.

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