【Objective】 To evaluate the competence of modified extractive method in removing myelin sheath

thereby seek a kind of effective extractive method to prepare acellular nerve scaffold. 【Methods】 Twelve adult SD rats weighing 230-280 g were used. The bilateral sciatic nerves were harvested and divided evenly into three groups according to preparation methods: control group were fresh nerve; Hudson group were treated by Triton X-200; sulfobetaine-10 (SB-10)

sulfobetaine-16 (SB-16) and modified group were processed by Triton X-200

SB-10

SB-16

sodium deoxycholate (SDC) and peroxyacetic acid (PAA). HE

toluidine blue

and transmission electron microscope (TEM) were used to monitor the efficiency of extractive method by assess the degrees of decellularization

demyelination and integrity of basal lamina tube. 【Results】 In Hudson group

HE staining showed that cell nucleis totally disappeared. Toluidine blue staining revealed concentrically ringed myelin sheath debris

stratiform structure of myelin sheath was distinct apart from a little incompact and axons were still visible in TEM detection. Modified group presented the same HE results

while Toluidine blue staining and TEM showed sample exhibited irregularly porous structure

myelin sheath and axons were completely removed and basal lamina tubes were integrally preserved

when compared to Hudson group.【Conclusion】 Modified extractive method exhibited a good efficiency in decellularization and demyelination

and provided protection for extracellular matrix.