【Objective】 RNAi technique was applied to inhibit the expression of the targeted Cdc6 in the tongue cancer cells to observe the proliferation and apoptosis of the tongue cancer cells. 【Methods】 The experiment divides to four groups: CON

NC

KD1

and KD2. Lentiviral vector transfects CAL-27 cells

real-time PCR and Western blot were used to examine the knock-down effects of the RNA interference on the levels of expression of Cdc6 mRNA and protein. The MTT method was used to examine the growth levels of CAL-27 cells. Flow cytometry was used to examine the cell cycle changes and apoptosis rates. 【Results】 The real-time PCR and Western blot results showed that KD1 and KD2 targets had significant knock-down effects on the expression of Cdc6. Compared with the NC group

the knockdown efficiencies of KD1 and KD2 on the Cdc6 gene were 33.68% and 35.79%

the levels of expression of Cdc6 protein were reduced by 44.44% and 66.67%

respectively. The cell growth levels in the KD1 and KD2 groups were inhibited

the cell growth inhibition rate in the KD1 and KD2 groups were 34.78% and 36.52% . Compared with the NC group

the apoptosis rates of CAL-27 cells were raised by 57.55% and 83.81% in the KD1 and KD2 groups. 【Conclusions】 The Cdc6-targeted lentiviral vector RNAi can effectively inhibit DNA replication of CAL-27 cells

preventing the proliferation of tongue cancer cells.