【Objective】 To compare effect of Macaca mulatta IL-4 and variant IL-4 (vIL-4) on transforming peripheral blood monocytes to dendritic cells. 【Methods】 Macaca mulatta PBMCs were isolated by Ficoll/Hypaque density gradient centrifugation

then magnetic activated cell sorter (MACS) CD3 MicroBead Kit (non-human primate) was used to negatively selected non-CD3+ cells

after that

peripheral blood monocytes were separated by attaching to plate wall and analyzed by flow cytometry to identify. Monocyte enriched cells were cultured by grouping as medium alone

IL-4+GM-CSF and vIL-4+ GM-CSF. Cells were collected and surface marked with CD14

CD1a

CD11c

and CD83 then analyzed by flow cytometry.【Result】 Up to 90% inactive peripheral blood monocytes were separated by the three steps used. Data of flow cytometry for cultured cells showed that compared to negative control group

CD14 expression from both IL-4+GM-CSF group and vIL-4+GM-CSF group were suppressed

but CD1a

CD11c

and CD83 markers were distinctly up-expressed

and no significant difference became obvious between those two groups. 【Conclusion】 Same with IL-4

vIL-4 transformed peripheral blood monocytes to dendritic cells.