【Objective】 To investigate the DV2 (Dengue virus type 2)-induced autophagy in the human endothelial cell line EAhy926. 【Methods】 Flow cytometric analysis was used to detect the percentage of autophagic cells of EAhy926 and the mean fluorescent intensity (MFI) of autophagic vacuoles after DV2 infection for various time periods (12 h

24 h

36 h

and 48 h)

at the same time

the Eahy926 cells in control groups were collected for detection. All experiments were repeated for 5 times. The variance analysis and the t test were used for analysis. Values were considered statistically significant at P < 0.05. 【Results】 Compared with the control groups

the percentage of autophagic cells of EAhy926 and the MFI of the autophagic vacuoles increased in DV2 infection groups (P < 0.05). The differential value between control groups and DV2 infection groups was most significant in 48 h. In different times (12 h

24 h

36 h

and 48 h)

there were (70 ± 7)%

(72 ± 6)%

(71 ± 8)%

and (69 ± 10)% of autophagic cells in control groups respectively

while in DV2 infection groups

there were (75 ± 3)%

(78 ± 3)%

(77 ± 5)%

and (80 ± 7)% of autophagic cells

respectively. Accordingly

the MFI of autophagic vacuoles in control groups was 36.4 ± 3.4

37.0 ± 3.3

35.8 ± 2.3

and 34.4 ± 3.2 in the four timepoints respectively

while in DV2 infection groups

the MFI of autophagic vacuoles was 41.1 ± 2.3

45.0 ± 4.8

41.3 ± 3.3

and 44.5 ± 7.5

respectively. 【Conclusions】 DV2 can induce the autophagy of EAhy926.